Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Francisella tularensis strain typing using multiple-locus, variable-number tandem repeat analysis.

J Farlow1, K L Smith, J Wong

  • 1Department of Biological Sciences, Northern Arizona University, Flagstaff, Arizona 86011-5640, USA.

Journal of Clinical Microbiology
|August 30, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction to: Genomic characterization of three novel Basilisk-like phages infecting Bacillus anthracis.

BMC genomics·2018
Same author

Genomic characterization of three novel Basilisk-like phages infecting Bacillus anthracis.

BMC genomics·2018
Same author

Association of Neisseria cinerea with ocular infections in paediatric patients.

The Journal of infection·1998
Same author

Inhibition of cellular proliferation by the Wilms tumor suppressor WT1 requires association with the inducible chaperone Hsp70.

Genes & development·1998
Same author

Unusual features of the Drosophila melanogaster telomere transposable element HeT-A are conserved in Drosophila yakuba telomere elements.

Proceedings of the National Academy of Sciences of the United States of America·1998
Same author

Current status of pharyngolaryngo-esophagectomy and pharyngogastric anastomosis.

Head & neck·1998

A new multilocus variable-number tandem repeat analysis (MLVA) system effectively types Francisella tularensis, the cause of tularemia. This method aids in tracking outbreaks and understanding the pathogen

Area of Science:

  • Microbiology
  • Genetics
  • Epidemiology

Background:

  • Francisella tularensis is the causative agent of tularemia, a zoonotic disease prevalent in the Northern Hemisphere.
  • Accurate typing methods are crucial for understanding the epidemiology and genetic diversity of F. tularensis.

Purpose of the Study:

  • To develop and evaluate a multilocus variable-number tandem repeat (MLVA) typing system for Francisella tularensis.
  • To assess the utility of MLVA for differentiating F. tularensis isolates and identifying outbreak sources.

Main Methods:

  • Genomic sequence analysis of F. tularensis to identify potential variable-number tandem repeat (VNTR) loci.
  • Development of PCR assays for six VNTR loci.
  • Typing of 56 F. tularensis isolates from various US states and the live vaccine strain.

Related Experiment Videos

  • Cluster analysis to evaluate genetic relationships and compare with existing biovar classification.

    • Main Results:

    • The MLVA system detected variation at six VNTR loci, with allele numbers ranging from 2 to 20.
    • Cluster analysis revealed two genetically distinct groups, aligning with the current biovar classification of F. tularensis.
    • Biovar B isolates exhibited less genetic diversity compared to biovar A isolates.
    • Seven isolates from a recent Arizona epizootic were identical across all VNTR loci, suggesting a common infection source.

    Conclusions:

    • The developed VNTR markers are effective for identifying F. tularensis isolates at the taxonomic level of biovars.
    • MLVA is a valuable tool for rapid characterization and identification of F. tularensis outbreak isolates.
    • Future development of reference databases using MLVA will enhance outbreak tracking and global genetic relationship studies.