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Updated: Aug 4, 2026
Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
Published on: March 8, 2012
Novel method for detection, typing, and quantification of human papillomaviruses in clinical samples
K W Hart1, O M Williams, N Thelwell
1Section of Infection and Immunity, Department of Medicine, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XX, United Kingdom. HartKW@cf.ac.uk
Abstract:
We report the development of a novel detection and typing methodology for human papillomaviruses (HPV) based on real-time PCR with the self-probing fluorescent primers known as Scorpions. This technique is quick, simple, specific, sensitive, and capable of estimating viral load per cell. We report the results of over 100 typing reactions performed on cell lines, biopsies, and cervical cytobrush samples which, when compared to the current reference HPV detection and typing technique, present a kappa value of 0.89. We further report preliminary data suggesting a relationship between viral load per cell and grade of cervical disease.
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