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Biofilm formation by Candida dubliniensis
G Ramage1, K Vande Walle, B L Wickes
1Department of Microbiology, Division of Infectious Diseases, The University of Texas Health Science Center at San Antonio, San Antonio, Texas 78245, USA. ribot@uthscsa.edu
Journal of Clinical Microbiology
|August 30, 2001
Summary
Candida dubliniensis forms resilient biofilms on biomaterials, showing increased antifungal resistance. These biofilms, crucial for infection and colonization, present significant clinical challenges.
Area of Science:
- Mycology
- Medical Microbiology
- Biomaterials Science
Background:
- Candida dubliniensis is an opportunistic yeast linked to oropharyngeal candidiasis in HIV patients.
- Biofilm formation is key in candidiasis, altering yeast properties compared to planktonic cells.
Purpose of the Study:
- To develop in vitro models for Candida dubliniensis biofilms on biomaterials.
- To characterize the properties of C. dubliniensis biofilms and their antifungal susceptibility.
Main Methods:
- Formazan salt reduction assay for metabolic activity, microscopy (SEM, CLSM) for structural analysis.
- Biofilm formation on polystyrene and acrylic surfaces studied over 24-48 hours.
- Antifungal susceptibility testing of biofilm (sessile) versus planktonic cells against fluconazole and amphotericin B.
Main Results:
- C. dubliniensis biofilm formation involves adherence, growth, and maturation over 24-48 hours.
- Serum and saliva enhanced initial attachment and biofilm development.
- Mature biofilms exhibit a dense network of yeast and hyphal cells within exopolymeric material, with spatial heterogeneity and water channels.
- Sessile C. dubliniensis cells showed significantly increased resistance to fluconazole and amphotericin B compared to planktonic cells.
Conclusions:
- Candida dubliniensis forms complex, resistant biofilms on biomaterials.
- Biofilm formation is critical for C. dubliniensis colonization and infection, posing clinical challenges.
- Understanding C. dubliniensis biofilms is essential for managing infections, particularly in immunocompromised individuals.