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Detection of rpoB mutations in Mycobacterium tuberculosis by biprobe analysis
K J Edwards1, L A Metherell, M Yates
1Molecular Biology Unit, Virus Reference Division, Central Public Health Laboratory, Colindale, London, NW9 5HT, United Kingdom.
Journal of Clinical Microbiology
|August 30, 2001
Abstract:
A biprobe assay utilizing LightCycler technology was developed to detect rifampin resistance-associated gene mutations in the Mycobacterium tuberculosis rpoB gene. Three biprobes detected all mutations present in the 46 rifampin-resistant isolates. Wild-type sequences were correctly identified in each case. The method was reproducible, accurate, and easy to use.