Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Gene expression following acute morphine administration.

A V Loguinov1, L M Anderson, G J Crosby

  • 1Department of Nutritional Sciences and Toxicology, University of California, Berkeley, California, USA.

Physiological Genomics
|August 30, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

HER2DX in HER2-positive inflammatory breast cancer: correlative insights and comparative analysis with noninflammatory breast cancers.

ESMO open·2025
Same author

Dietary restraint: what's the harm? A review of the relationship between dietary restraint, weight trajectory and the development of eating pathology.

Clinical obesity·2016
Same author

Social functioning and facial expression recognition in children with neurofibromatosis type 1.

Journal of intellectual disability research : JIDR·2016
Same author

Participation as a leader in immersion weight loss treatment: a 1-year follow-up study.

Clinical obesity·2015
Same author

Comparison of flavor changes in cooked-refrigerated beef, pork and chicken meat patties.

Meat science·2011
Same author

miR-23b targets proline oxidase, a novel tumor suppressor protein in renal cancer.

Oncogene·2010
Same journal

Regulation of RGS2 is an important piece to the puzzle of syncytiotrophoblast dysfunction in preeclampsia.

Physiological genomics·2026
Same journal

Maternal Estradiol Impacts Fetal HPA Axis and Pulmonary Transcriptomes in Sheep.

Physiological genomics·2026
Same journal

OBESITY AS AN EPIGENETIC CONTINUUM: DEVELOPMENTAL ROOTS, ADIPOSE REMODELING, AND THE LIMITS OF REVERSIBILITY.

Physiological genomics·2026
Same journal

<i>Akkermansia muciniphila</i>: a member of the gut microbiota that modulates intestinal stem cell function.

Physiological genomics·2026
Same journal

Differential gene transcription following intravenous injection of air bubbles in rats with varying resistance to decompression sickness.

Physiological genomics·2026
Same journal

Genomic informational field theory to identify genetic associations of a complex trait using a small sample size.

Physiological genomics·2026
See all related articles

A single dose of morphine alters gene expression in the brain and spinal cord, affecting mitochondrial and cytoskeleton proteins. These changes, primarily downregulations, are temporary and reversed by naloxone.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Pharmacology

Background:

  • Neurotropic drug responses involve neuroplasticity and gene expression changes.
  • Neuronal gene expression can be altered by even a single drug administration.

Purpose of the Study:

  • To investigate the extent of gene expression changes in the nervous system after a single morphine injection.
  • To identify specific gene groups affected by morphine and explore potential mediating pathways.

Main Methods:

  • Gene expression was monitored using cDNA microarray in the medial striatum and lumbar spinal cord after a single morphine injection.
  • Differentially expressed genes were detected using robust linear regression (MM-estimator) with simultaneous prediction confidence intervals.
  • Cluster analysis was employed to group affected genes.

Related Experiment Videos

Main Results:

  • A single morphine injection significantly altered the expression of genes related to mitochondrial respiration and cytoskeleton proteins.
  • These gene expression changes, predominantly downregulations, were observed in both the medial striatum and lumbar spinal cord.
  • The observed transitory changes were prevented by the coadministration of the opioid antagonist naloxone.

Conclusions:

  • Microarray analysis is a valuable tool for characterizing the effects of known substances on the nervous system.
  • Morphine's acute effects involve alterations in mitochondrial and cytoskeletal gene expression, suggesting a novel pathway for its activity.