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Related Experiment Videos

Multiple gastrointestinal atresias result from disturbed morphogenesis.

L Fourcade1, H Shima, E Miyazaki

  • 1Children's Research Centre, Our Lady's Hospital for Sick Children, Crumlin, Dublin, Ireland.

Pediatric Surgery International
|August 31, 2001
PubMed
Summary

Multiple gastrointestinal atresias (MGA) in rats result from early gestation adriamycin exposure, indicating a malformative, not ischemic, process. This study highlights MGA

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Area of Science:

  • Developmental biology
  • Toxicology
  • Gastroenterology

Background:

  • Multiple gastrointestinal atresias (MGA) represent a significant portion of intestinal atresias, with ongoing debate regarding their cause.
  • The prevailing theories suggest either multiple ischemic events or a malformative process during fetal development.

Purpose of the Study:

  • To investigate the incidence and histopathologic features of MGA in a rat model.
  • To elucidate the pathogenesis of MGA by examining the effects of prenatal adriamycin exposure.

Main Methods:

  • Timed-pregnant Sprague-Dawley rats were administered adriamycin (1.75 mg/kg) across nine different gestational protocols.
  • Fetuses were delivered via cesarean section on day 21, and their digestive tracts were examined macroscopically and microscopically.

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  • MGA incidence was specifically analyzed in litters exposed on gestational days 7, 8, and 9.

    • Main Results:

    • Adriamycin exposure on gestational days 7, 8, and 9 induced MGA in 94% of affected newborns (80/86 fetuses).
    • Newborns with MGA exhibited a high frequency of associated congenital anomalies.
    • Histological examination revealed varying degrees of villous hyperplasia in atretic segments, sometimes with intraluminal material.

    Conclusions:

    • Prenatal adriamycin exposure during critical early gestation periods reliably induces MGA in rats.
    • The findings strongly support a malformative etiology for MGA, rather than an ischemic cause.
    • This rat model provides valuable insights into the developmental origins of MGA and associated anomalies.