ik3-1/Cables is associated with Trap and Pctaire2
T Yamochi1, I Nishimoto, T Okuda
1Department of Pharmacology, KEIO University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan.
Biochemical and Biophysical Research Communications
|August 31, 2001
Summary
The study identifies mouse Trap as a binding partner for ik3-1/Cables, potentially linking Pctaire kinases with cell cycle regulators like cdk3 and cdk5 in neuronal development.
Area of Science:
- Molecular and Cellular Neuroscience
- Cell Cycle Regulation
Background:
- ik3-1/Cables protein functions in cell cycle regulation and neuronal development.
- In neurons, ik3-1/Cables may act as an adaptor, linking c-abl and cdk5 to support neurite growth.
Purpose of the Study:
- To identify proteins interacting with ik3-1/Cables.
- To investigate the potential functional connections between ik3-1/Cables, Pctaire kinases, and Trap in neuronal cells.
Main Methods:
- Cloning of mouse Trap (tudor repeat associator with Pctaire 2) cDNA.
- Co-immunoprecipitation assays to detect protein interactions.
- In vitro kinase assays to assess kinase activity.
Main Results:
- Mouse Trap interacts with ik3-1/Cables via its C-terminal tudor repeat domains (4 and 5).
- The N-terminal domain of ik3-1/Cables is crucial for Trap binding.
- ik3-1/Cables co-immunoprecipitated with Pctaire 2, suggesting a complex formation.
Conclusions:
- ik3-1/Cables interacts with mouse Trap, a novel binding partner.
- These findings suggest a potential role for ik3-1/Cables in connecting the Pctaire kinase family and Trap with cdk3 or cdk5, impacting neuronal function.
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