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Updated: Oct 4, 2026
11:58
A Simple, Rapid, and Quantitative Assay to Measure Repair of DNA-protein Crosslinks on Plasmids Transfected into Mammalian Cells
Published on: March 5, 2018
Hydrolysis of plasmid DNA catalyzed by Co(III) complex of cyclen attached to polystyrene
1Artzyme Biotech Corporation, 403-1, Silim-2-Dong, Seoul 151-012, South Korea.
Bioorganic & Medicinal Chemistry Letters
|August 31, 2001
Abstract:
Reactivity of the Co(III) complex of cyclen (CoCyc) in the hydrolytic cleavage of supercoiled pUC18 DNA leading to the formation of the corresponding open circular form was enhanced by >200 times upon attachment of CoCyc to cross-linked polystyrenes. Thus, half-lives as short as 40 min were achieved by the resin-based CoCyc in cleavage of the supercoiled DNA at 4 degrees C.
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