Related Experiment Video
Updated: Oct 4, 2026
A Murine Model of Fetal Exposure to Maternal Inflammation to Study the Effects of Acute Chorioamnionitis on Newborn Intestinal Development
Published on: June 24, 2020
The effect of amnio-allantoic fluid pH on the intestines: an experimental study in the chick embryo gastroschisis
T Kanmaz1, A Yağmurlu, T Aktuğ
1Department of Pediatric Surgery, Ankara University Faculty of Medicine, Ankara, Turkey.
Insights
Alkalizing the amnio-allantoic fluid (AAF) in a gastroschisis model significantly reduced intestinal damage. This suggests AAF pH adjustment may be a promising treatment to prevent intestinal injury in gastroschisis.
Area of Science:
- Developmental Biology
- Surgical Research
- Neonatal Medicine
Background:
- Gastroschisis leads to intestinal damage due to prolonged exposure to amniotic fluid.
- Intestinal changes include edema, thickening, fibrous coating, and adhesions.
- The pH of amnio-allantoic fluid (AAF) is a potential factor influencing this damage.
Purpose of the Study:
- To evaluate the effect of amnio-allantoic fluid (AAF) pH on intestinal damage in a chick embryo gastroschisis model.
- To investigate if alkalizing the AAF can mitigate intestinal injury associated with gastroschisis.
Main Methods:
- A chick embryo model of gastroschisis was established on day 14 of incubation.
- Seventy eggs were divided into five groups: preliminary study, AAF control, just gastroschisis, gastroschisis with placebo, and gastroschisis with sodium bicarbonate (NaHCO3) pretreatment.
- The sodium bicarbonate group received alkalized AAF for four days.
Main Results:
- Macroscopic and microscopic intestinal damage was significantly reduced in the gastroschisis group pretreated with sodium bicarbonate (GPS) compared to the just gastroschisis (JG) and gastroschisis with placebo (GPP) groups.
- Alkalization of the AAF demonstrated a protective effect on the intestines.
Conclusions:
- Pretreatment involving the alkalization of amnio-allantoic fluid effectively prevented intestinal damage in the gastroschisis model.
- AAF pH adjustment presents a potential, simple alternative to amniotic fluid exchange for treating human fetuses with gastroschisis.
Background/Purpose:
Prolonged exposure to amniotic fluid causes the intestinal changes such as serosal edema, thickening, fibrous coating, and adhesions in gastroschisis. The effect of amnio-allantoic fluid (AAF) pH on intestines was evaluated using a chick embryo gastroschisis model.
Methods:
Seventy fertile eggs were divided into 5 groups: preliminary study (PS, n = 20), AAF control (AAC, n = 10), just gastroschisis (JG, n = 10), gastroschisis pretreated with placebo (GPP, n = 15), and gastroschisis pretreated with sodium bicarbonate (GPS, n = 15). The PS group was also divided into 2 subgroups to determine the biochemical differences between the amniotic and the allantoic fluid. Gastroschisis was created surgically at the 14th day of incubation. In GPS group, 8.4% NaHCO(3) solution (0.1 mL/100 mg/d) was instilled into the AAF for 4 days.
Results:
A significant decrease in intestinal damage was observed both macroscopically and microscopically in the group GPS compared with the JG and GPP groups.
Conclusions:
Pretreatment with alkalization of AAF prevented intestinal damage because of gastroschisis. Alkalization could be a simple alternative for pretreatment with amniotic fluid exchange for human fetuses with gastroschisis.
More Related Videos
09:30Dissection and Explant Culture of Murine Allantois for the In Vitro Analysis of Allantoic Attachment
Published on: January 13, 2018
09:53The Preparation of Chicken Ex Ovo Embryos and Chorioallantoic Membrane Vessels as In Vivo Model for Contrast-Enhanced Ultrasound Imaging and Microbubble-Mediated Drug Delivery Studies
Published on: February 9, 2021
