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Updated: Aug 11, 2026

Plunge Freezing: A Tool for the Ultrastructural and Immunolocalization Studies of Suspension Cells in Transmission Electron Microscopy
Published on: May 5, 2017
Effect of freezing on the length of the penis in Nucella lapillus (L.)
1FRS Marine Laboratory, PO Box 101, Victoria Road, Aberdeen, UK AB11 9DB. minchina@marlab.ac.uk
Abstract:
When quantifying imposex in Nucella lapillus, two indices are used: the Vas Deferens Sequence Index (VDSI) and the Relative Penis Size Index (RPSI). Freezing and thawing increase the length of the penis in both male and female Nucella lapillus. In the population studied, this had no significant effect on the RPSI, but was potentially an important source of additional variance in the estimation of the mean penis length.
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