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Related Experiment Videos

Characterization of equine E-selectin.

J F Hedges1, C D Demaula, B D Moore

  • 1Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.

Immunology
|September 1, 2001
PubMed
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Equine E-selectin, crucial for white blood cell movement during inflammation, was characterized. Horses share closer E-selectin gene relations with humans than sheep or deer, with higher expression in horses contributing to their endotoxemia sensitivity.

Area of Science:

  • Immunology
  • Molecular Biology
  • Comparative Genomics

Background:

  • E-selectin expression on activated endothelium is vital for leukocyte extravasation during inflammation.
  • E-selectin remains poorly characterized in several animal species, including horses.
  • Understanding equine E-selectin is critical for investigating inflammatory conditions in horses.

Purpose of the Study:

  • To sequence and compare equine, cervid, and ovine E-selectin genes.
  • To characterize the expression and function of equine E-selectin.
  • To investigate the relationship between equine E-selectin and human E-selectin.

Main Methods:

  • Sequencing of E-selectin genes from purified equine, cervid, and ovine pulmonary artery endothelial cells (ECs).
  • Phylogenetic and amino acid sequence comparisons.

Related Experiment Videos

  • Utilized human E- and P-selectin-specific monoclonal antibodies to characterize equine E-selectin expression and function.

    • Main Results:

    • Phylogenetic analysis revealed that equine E-selectin is more closely related to human E-selectin than to ruminant (bovine, cervid, ovine) E-selectins.
    • Equine E-selectin expression was more readily induced by lipopolysaccharide (LPS) in equine ECs compared to human ECs.
    • Induced equine E-selectin supported neutrophil adhesion and activation, mirroring findings in endotoxemia.

    Conclusions:

    • Equine E-selectin shares a closer evolutionary relationship with human E-selectin.
    • Enhanced inducibility and function of equine E-selectin in response to LPS correlate with horses' sensitivity to endotoxemia and septic shock.
    • This study provides foundational characterization of equine E-selectin, relevant for veterinary immunology and comparative studies.