Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Lineage-switching by pluripotent cells derived from adults].

F Dieterlen-Lièvre1

  • 1Institut d'Embryologie cellulaire et moléculaire du CNRS et du College de France, 49 bis, avenue de la Belle Gabrielle, 94736 Nogent-sur-Marne. dieterle@infobiogen.fr

Journal De La Societe De Biologie
|September 4, 2001
PubMed
Summary

Recent research reveals unexpected stem cell plasticity, enabling fate switches under specific conditions. This discovery holds promise for cell therapy, but controlling transplanted stem cell proliferation remains a key challenge.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Heart targeting of retroviral expression in avian embryos: a species-independent phenomenon.

Roux's archives of developmental biology : the official organ of the EDBO·2017
Same author

In vivo diversification and migrations of chick embryo heart muscle cells: a morphometric analysis with ALV- and SNV-based non-replicative vectors.

Development genes and evolution·2013
Same author

Hemangioblast commitment in the avian allantois: cellular and molecular aspects.

Developmental biology·2002
Same author

[Embryogenesis of the blood system].

Therapie·2001
Same author

Ontogeny of the endothelial system in the avian model.

Advances in experimental medicine and biology·2000
Same author

Tracing the progeny of the aortic hemangioblast in the avian embryo.

Developmental biology·2000

Area of Science:

  • Developmental Biology
  • Stem Cell Biology
  • Cell Therapy

Background:

  • Embryonic morphogenesis involves germ layer formation during gastrulation, with each layer typically giving rise to specific lineages.
  • Historically, fate switches between germ layers were considered impossible, but recent findings demonstrate significant plasticity in stem cells.
  • Stem cells, characterized by self-renewal and pluripotency, maintain tissue homeostasis and were previously thought to be lineage-restricted.

Purpose of the Study:

  • To review recent discoveries regarding stem cell plasticity and its implications for cell therapy.
  • To highlight novel methods for stem cell isolation and identification.
  • To discuss the role of specific genes and experimental conditions in inducing stem cell fate switching.

Main Methods:

Related Experiment Videos

  • Identification and isolation of stem cells using Hoechst 33342 dye efflux, defining a 'side population' (SP) with weak fluorescence.
  • Analysis of the multidrug resistance (mdr) gene's role in dye efflux and toxin extrusion.
  • Investigation of the Pax7 gene's role in muscle stem cell commitment and regeneration.

Main Results:

  • Unexpected plasticity has been observed in stem cells, allowing differentiation into various lineages, including neural stem cells into diverse derivatives.
  • The Pax7 gene acts as a commitment factor; its absence in Pax7-/- mice prevents muscle stem cell specification, redirecting SP cells towards hematopoietic lineages.
  • Stem cell fate switching often requires drastic experimental conditions, such as sublethal irradiation combined with genetic deficiencies.

Conclusions:

  • Recent findings underscore the plasticity of stem cells within their respective tissues, challenging previous notions of lineage restriction.
  • Inducing phenotype switching in stem cells necessitates specific experimental manipulations and a deeper understanding of niche factors.
  • Future progress in stem cell research, particularly for therapeutic applications, depends on identifying niche-specific factors and controlling transplanted stem cell proliferation.