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Updated: Apr 7, 2026
Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy
Published on: August 20, 2018
[Immobilization of chloroperoxidase from Serratia marcescens]
Iu V Preobrazhenskaia1, V N Burd', A I Voskoboev
1Grodno State University, Ozheshko ul, 22, Grodno, 230012 Belarus.
Abstract:
A bacterial non-heme chloroperoxidase from Serratia marcescens W 250 was immobilized in calcium-alginate gel. Methods for stabilization of the immobilized enzyme were developed, and some kinetic parameters of the immobilized preparations were determined. The enzyme encapsulated into the gel granules in the presence of potassium ferricyanide followed by treatment with glutaraldehyde demonstrated the highest stability under the reaction conditions.
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