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Related Experiment Videos

[Assay in high throughput screening].

K Arai1

  • 1Business Strategy Division, Taisho Pharmaceutical Co. Ltd., Saitama 330-8530, Japan. s16751@ccm.taisho.co.jp

Nihon Yakurigaku Zasshi. Folia Pharmacologica Japonica
|September 4, 2001
PubMed
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High Throughput Screening (HTS) advances drug discovery through efficient, miniaturized assays. Homogeneous assay technologies and quality metrics like the Z'-factor are key to successful lead compound identification.

Area of Science:

  • Drug discovery and medicinal chemistry
  • Biotechnology and assay development
  • Pharmacology and screening technologies

Context:

  • High Throughput Screening (HTS) is crucial for identifying potential drug candidates.
  • Efficiency in sample preparation, assay procedures, automation, and data management is paramount in HTS.
  • Miniaturization using high-density microplates (384 and 1536 wells) increases throughput and reduces costs.

Purpose:

  • This review focuses on assay technology and efficiency in High Throughput Screening (HTS).
  • To explore trends in HTS, particularly assay miniaturization and homogeneous assay systems.
  • To highlight methods for evaluating assay quality, such as the Z -factor.

Summary:

  • Homogeneous assay systems, eliminating separation steps, are effective for miniaturized HTS.

Related Experiment Videos

  • Common homogeneous assays include scintillation proximity assay (SPA), fluorescence energy transfer (HTRF, LANCE), and fluorescence polarization (FP).
  • Reporter gene assays, cell proliferation assays, and intracellular Ca2+ influx measurements are adaptable to homogeneous HTS formats.

    • Impact:

    • Miniaturization and homogeneous assays enhance the efficiency and cost-effectiveness of drug discovery.
    • The adoption of advanced assay technologies accelerates the identification of lead compounds.
    • Robust assay quality assessment using metrics like the Z -factor ensures reliable screening results.