Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Bovine melanocortin receptor 4: cDNA sequence, polymorphisms and mapping.

A Haegeman1, F Coopman, K Jacobs

  • 1Department of Animal Nutrition, Genetics, Breeding and Ethology, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium.

Animal Genetics
|September 5, 2001
PubMed
Summary

Researchers cloned the bovine melanocortin 4 receptor (MC4R) gene, identifying two key genetic variations. One variation, alanine to threonine substitution at position 172, was found predominantly in Red Holstein cattle.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[The skin and mucosa of the head and neck area. Chewing how does that work again?]

Nederlands tijdschrift voor tandheelkunde·2026
Same author

[The skin and mucosa of the head and neck area...What was the innervation again?]

Nederlands tijdschrift voor tandheelkunde·2025
Same author

[Salivary glands: what was innervation again?]

Nederlands tijdschrift voor tandheelkunde·2025
Same author

A survey of direct-to-consumer genetic testing of small animals in European laboratories.

The Journal of small animal practice·2025
Same author

<i>Morchella capensis</i> sp. nov., the Fynbos morel-the first description of an endemic <i>Morchella</i> species from Africa.

Canadian journal of microbiology·2025
Same author

Imaging the development of the human craniofacial arterial system - an experimental study.

Pediatric radiology·2024

Area of Science:

  • Genetics
  • Animal Science
  • Molecular Biology

Background:

  • The melanocortin 4 receptor (MC4R) plays a crucial role in regulating energy balance and body weight.
  • Understanding genetic variations in MC4R can provide insights into metabolic traits in cattle.

Purpose of the Study:

  • To clone and characterize the bovine MC4R gene.
  • To identify genetic variations within the bovine MC4R and their distribution across different cattle breeds.

Main Methods:

  • Cloning and sequencing of the bovine MC4R cDNA.
  • Comparative sequence analysis with human, pig, and rat homologues.
  • Radiation hybrid mapping to determine gene location.
  • Single-stranded conformation polymorphism (SSCP) and sequencing to identify nucleotide changes.

Related Experiment Videos

  • Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to screen cattle breeds.

    • Main Results:

    • The bovine MC4R shares high DNA and protein sequence identity with homologues from other species.
    • Two nucleotide substitutions were identified: Val145Ala and Ala172Thr.
    • The Ala172Thr substitution (allele A) was detected almost exclusively in Red Holstein cattle among 13 screened breeds.

    Conclusions:

    • The bovine MC4R gene has been successfully cloned and mapped.
    • A specific MC4R allele (Ala172Thr) is associated with Red Holstein cattle, suggesting potential breed-specific genetic markers.
    • Further research could explore the functional implications of these MC4R variations on cattle traits.