Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Evaluation of the OSIRIS video reader system for disk diffusion susceptibility test reading.

M A Sánchez1, B Sánchez del Saz, E Loza

  • 1Servicio de Microbiología, Hospital Ramón y Cajal, 28034-Madrid, Spain.

Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases
|September 5, 2001
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Antechodynamics and Antechokinetics: Dynamics and Kinetics of Antibiotic Resistance Biomolecules.

Biomolecules·2025
Same author

Selection versus transmission: Quantitative and organismic biology in antibiotic resistance.

Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases·2024
Same author

Coexistence of genetically modified seed production and organic farming in Chile.

GM crops & food·2022
Same author

Simulating the impact of non-pharmaceutical interventions limiting transmission in COVID-19 epidemics using a membrane computing model.

microLife·2021
Same author

Evolutionary Pathways and Trajectories in Antibiotic Resistance.

Clinical microbiology reviews·2021
Same author

Antimicrobial activity of ceftolozane-tazobactam against Enterobacterales and Pseudomonas aeruginosa recovered during the Study for Monitoring Antimicrobial Resistance Trends (SMART) program in Spain (2016-2018).

Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia·2021

The OSIRIS video-assisted system accurately reads inhibition zones for disk diffusion susceptibility testing. It shows high performance, especially with MRSA, making it a useful tool for clinical laboratories.

Area of Science:

  • Clinical microbiology
  • Antimicrobial resistance
  • Diagnostic technology

Background:

  • Disk diffusion susceptibility testing is a standard method for determining bacterial susceptibility to antibiotics.
  • Accurate interpretation of inhibition zone sizes is crucial for effective antimicrobial therapy.
  • Automated or semi-automated systems can improve the efficiency and reproducibility of this testing.

Purpose of the Study:

  • To evaluate the performance of the OSIRIS video-assisted reading system.
  • To compare OSIRIS system readings with conventional manual readings for disk diffusion tests.
  • To assess the system's utility in identifying antimicrobial resistance mechanisms.

Main Methods:

  • Prospective collection of 119 clinical isolates, including Enterobacteriaceae with ESBL/IRT, MRSA, and VRE.

Related Experiment Videos

  • Standard National Committee for Clinical Laboratory Standards disk-diffusion technique was employed.
  • OSIRIS system inhibition zone readings were compared against manual readings as the reference.

    • Main Results:

    • Essential agreement between OSIRIS and manual readings was high (91.6% for routine, 94.8% for resistant isolates).
    • Overall interpretation agreement was also high, slightly better for routine isolates (95.5%) than resistant ones (93.2%).
    • The system demonstrated high performance with MRSA but was affected by poor growth in enterococci; ESBL presence influenced measurements.

    Conclusions:

    • The OSIRIS system is a valuable tool for reading and interpreting inhibition zone sizes in disk diffusion susceptibility testing.
    • Its performance is comparable to manual methods, with potential for improved efficiency.
    • The system aids in the accurate interpretation of antimicrobial susceptibility, supporting clinical decision-making.