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Updated: Jul 22, 2026
Isolating And Immunostaining Lymphocytes and Dendritic Cells from Murine Peyer's Patches
Published on: March 17, 2013
Formation of Peyer's patches
1Swiss Institute for Experimental Cancer Research & Institute of Biochemistry, University of Lausanne, CH-1066, Epalinges, Switzerland. daniela.finke@isrec.unil.ch
Insights
Peyer
Area of Science:
- Immunology and developmental biology, focusing on gut-associated lymphoid tissues.
Background:
- Peyer's patches (PPs) are crucial for gut immunity.
- Their formation involves intricate crosstalk between gut epithelium, mesenchyme, and immune cells.
Purpose of the Study:
- To elucidate the molecular signals governing Peyer's patch development.
- To understand the ontogeny of gut-associated lymphoid tissues.
Main Methods:
- Utilized knockout mouse models to investigate genetic requirements.
- Analyzed developmental stages around embryonic day 15.5.
Main Results:
- Identified key signaling molecules essential for Peyer's patch anlage formation.
- Highlighted the roles of tumor necrosis factor family members and chemokines.
Conclusions:
- Tumor necrosis factor family members and homeostatic chemokines are critical mediators of Peyer's patch ontogeny.
- These molecular signals orchestrate the complex interactions required for Peyer's patch formation.
Abstract:
Formation of Peyer's patches requires complex interactions between the gut epithelium, the mesenchyme, and bone-marrow-derived hematopoietic progenitors. The first Peyer's patches anlage appear around embryonic day 15.5, when the endoderm has undergone transition to a simple epithelium, the lymphatic vessels have reached the intestinal mucosa, and mesenchymal cells have started to form clusters. Recent data using knockout mice provide insight into the molecular nature of the signals that mediate Peyer's patch ontogeny. These include members of the tumor-necrosis factor family and homeostatic chemokines.
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