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Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions
Published on: June 28, 2018
Promoter targeting of chromatin-modifying complexes
A H Hassan1, K E Neely, M Vignali
1Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA.
Frontiers in Bioscience : a Journal and Virtual Library
|September 5, 2001
Summary
Chromatin remodeling complexes, including histone acetyltransferases (HATs) and SWI/SNF, are crucial for gene expression. Understanding their recruitment and interplay offers insights into gene control and human diseases like cancer.
Area of Science:
- Molecular Biology
- Epigenetics
- Gene Regulation
Background:
- Eukaryotic gene expression is regulated by multi-subunit complexes that overcome chromatin repression.
- Histone acetyltransferases (HATs) and histone deacetylases (HDACs) modify histone acetylation.
- The SWI/SNF complex alters chromatin conformation using ATP hydrolysis.
Purpose of the Study:
- To review promoter recruitment mechanisms for HATs and SWI/SNF complexes.
- To highlight the functional interplay between these chromatin-remodeling activities.
- To summarize corepressor recruitment of HDACs.
Main Methods:
- Literature review of studies on chromatin remodeling complexes.
- Analysis of genetic studies in yeast.
- Synthesis of recent data on promoter recruitment and functional interplay.
Main Results:
- Some gene promoters require modifications by both HATs and SWI/SNF complexes.
- Functional interplay between HATs and SWI/SNF activities is evident.
- Repressors and corepressors can recruit histone deacetylase complexes.
Conclusions:
- Understanding the recruitment of chromatin-modifying complexes is key to gene regulation.
- Interplay between HATs and SWI/SNF is critical for gene expression.
- Dysregulation of these complexes is linked to human diseases, including cancer.
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