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Updated: Aug 9, 2026

Pulse-chase Analysis of N-linked Sugar Chains from Glycoproteins in Mammalian Cells
Published on: April 28, 2010
UDP-sugar transporter implicated in glycosylation and processing of Notch
S Goto1, M Taniguchi, M Muraoka
1Genetic Strain Research Center, National Institute of Genetics, Mishima, Shizuoka-ken 411-8540, Japan. sgoto@mrc-lmb.cam.ac.uk
Abstract:
Glycosylation modifies protein activities in various biological processes. Here, we report the functions of a novel UDP-sugar transporter (UST74C, an alternative name for Fringe connection (Frc)) localized to the Golgi apparatus in cellular signalling of Drosophila. Mutants in the frc gene exhibit phenotypes resembling wingless and Notch mutants. Both Fringe-dependent and Fringe-independent Notch pathways are affected, and both glycosylation and proteolytic maturation of Notch are defective in mutant larvae. The results suggest that changes in nucleotide-sugar levels can differently affect Wingless and two distinct aspects of Notch signalling.
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