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Published on: July 6, 2019
Multiphoton confocal microscopy using a femtosecond Cr:forsterite laser
1Department of Electrical Engineering and Graduate Institute of Electro-Optical Engineering, National Taiwan University, Taipei, ROC.
Scanning
|September 6, 2001
Summary
A femtosecond Cr:forsterite laser, operating at 1,230 nm, effectively excites multiphoton fluorescence in biological probes and plant chlorophyll. This laser offers a promising alternative for advanced multiphoton microscopy applications.
Area of Science:
- Biophotonics
- Microscopy
- Laser Physics
Background:
- Multiphoton fluorescence microscopy offers deep tissue penetration and high resolution.
- Current systems often rely on Ti:sapphire lasers, which have limitations.
- Cr:forsterite lasers provide wavelengths suitable for biological imaging and possess advantageous properties.
Purpose of the Study:
- To evaluate the femtosecond Cr:forsterite laser as an excitation source for multiphoton scanning microscopy.
- To investigate its performance with common bioprobes and plant autofluorescence.
- To demonstrate its utility in imaging plant cellular structures.
Main Methods:
- Utilized a 1,230 nm femtosecond Cr:forsterite laser for multiphoton excitation.
- Performed photoluminescence spectrum measurements on bioprobes and chlorophyll in Arabidopsis thaliana.
- Acquired two-photon scanning microscopy images of plant leaf cells.
Main Results:
- Achieved efficient two-photon and three-photon fluorescence from various bioprobes.
- Observed strong chlorophyll autofluorescence at 673 nm and 728 nm in live plant leaves.
- Generated high-quality two-photon paradermal and cross-sectional images of palisade mesophyll cells.
Conclusions:
- The femtosecond Cr:forsterite laser is a viable and effective excitation source for multiphoton microscopy.
- Its performance with bioprobes and plant autofluorescence supports its use in biological imaging.
- This laser system facilitates detailed visualization of cellular structures in live plant tissues.
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