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Sampling Plans

Sampling Plans

Sampling is a crucial step in analytical chemistry, allowing researchers to collect representative data from a large population. Common sampling methods include random, judgmental, systematic, stratified, and cluster sampling.
Random sampling is a method where each member of the population has an equal chance of being selected for the sample. It involves selecting individuals randomly, often using random number generators or lottery-type methods. For example, when analyzing the properties of a...

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Related Experiment Video

Updated: Jul 16, 2026

Flow Cytometric Analysis of Particle-bound Bet v 1 Allergen in PM10

Flow Cytometric Analysis of Particle-bound Bet v 1 Allergen in PM10

Published on: November 19, 2016

A side-by-side comparison of sampling methods for settled, indoor allergens.

M Mansour¹, B P Lanphear, R Hornung

¹Cincinnati Lead and Allergen Sampling Study with Children's Hospital Medical Center, Department of Pediatrics, Ohio 45229, USA.

Environmental Research

|September 6, 2001

Summary

No single sampling method is best for measuring indoor allergens like dust mites and cat dander. The Cyclone and Mighty Mite samplers showed better precision for cat allergen, while Readivac II was better for dust mite allergen.

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Last Updated: Jul 16, 2026

Flow Cytometric Analysis of Particle-bound Bet v 1 Allergen in PM10

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Published on: November 19, 2016

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Published on: April 21, 2019

Symptom Assessment of Patients with Allergic Rhinitis Using an Allergen Exposure Chamber

Symptom Assessment of Patients with Allergic Rhinitis Using an Allergen Exposure Chamber

Published on: March 3, 2023

Area of Science:

Environmental Health
Allergy and Immunology
Occupational Health

Background:

Indoor allergen exposure is a significant risk factor for asthma development and exacerbation.
A lack of standardized sampling methods hinders accurate measurement of indoor allergen levels.
Assessing allergen exposure is crucial for understanding its relationship with allergic sensitization.

Purpose of the Study:

To compare the precision of three different sampling devices (Cyclone, Mighty Mite, Readivac II) for indoor allergen measurement.
To evaluate the association between measured allergen exposure and serum-specific IgE levels in asthmatic children.
To identify a standardized sampling method for indoor allergens.

Main Methods:

Seventy-two children aged 5-17 with asthma participated, residing in the same home for at least two years.
Composite floor dust samples were collected simultaneously using Cyclone, Mighty Mite, and Readivac II samplers.
Concentrations of Der f I (dust mite), Fel d I (cat), and Bla g I (cockroach) allergens were measured, alongside serum-specific IgE levels.

Main Results:

No significant differences in mean allergen concentrations were found across the three sampling methods.
Cat allergen (Fel d I) showed significant correlation with IgE for Cyclone and Mighty Mite samplers.
Dust mite allergen (Der f I) correlated significantly with IgE for Readivac II and Cyclone samplers. Cockroach allergen (Bla g I) did not correlate with IgE for any method. Cyclone and Mighty Mite were more precise for cat allergen than Readivac II.

Conclusions:

No single sampling method demonstrated superiority for measuring all indoor allergens.
The Cyclone sampler was a better predictor of cat allergen-specific IgE, while Readivac II was better for dust mite allergen-specific IgE.
Further research is needed to establish a universally standardized method for indoor allergen sampling.