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Nucleotide polymorphism in microcin V plasmids.

T Pinou1, M A Riley

  • 1Department of Ecology and Evolution, Yale University, New Haven, Connecticut 06520, USA.

Plasmid
|September 6, 2001
PubMed
Summary
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The microcin V gene cluster in Escherichia coli shows low DNA sequence polymorphism, differing from chromosomal DNA. This suggests the microcin V gene cluster has a distinct evolutionary path compared to its host.

Area of Science:

  • Microbiology
  • Genetics
  • Evolutionary Biology

Background:

  • The microcin V gene cluster is found on plasmids in Escherichia coli.
  • Understanding the genetic diversity of microbial genes is crucial for evolutionary studies.

Purpose of the Study:

  • To determine the DNA sequence polymorphism within the microcin V gene cluster.
  • To compare the polymorphism of the microcin V gene cluster with a chromosomal region in Escherichia coli.

Main Methods:

  • DNA sequencing of the microcin V gene cluster from 12 natural isolates of Escherichia coli.
  • DNA sequencing of a chromosomal region (trpORF2) from the same isolates.
  • Comparative analysis of sequence polymorphism levels and patterns.

Main Results:

Related Experiment Videos

  • The microcin V gene clusters exhibited low DNA sequence polymorphism (10 of 683 bp polymorphic).
  • Polymorphism patterns of the microcin V gene cluster differed significantly from the sequenced chromosomal region (trpORF2).

Conclusions:

  • The microcin V gene cluster has a distinct evolutionary history compared to the Escherichia coli host chromosome.
  • Plasmid-encoded genes may evolve independently from chromosomal genes.