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Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli
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Regulated ectopic expression and allelic-replacement mutagenesis as a method for gene essentiality testing in

F Fan1, R D Lunsford, D Sylvester

  • 1Antimicrobials and Host Defense CEDD, Collegeville, Pennsylvania 19426, USA. Frank_K_Fan@sbphrd.com

Plasmid
|September 6, 2001
PubMed

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In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
In vitro Mutagenesis01:16

In vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.