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Related Experiment Videos

Triplet-repeat microsatellites shared among hard and soft pines.

B L Kutil1, C G Williams

  • 1Graduate Genetics Program, Texas A&M University, College Station, Texas 77843-2135, USA.

The Journal of Heredity
|September 6, 2001
PubMed
Summary

Finding useful plant microsatellites is challenging. This study found that trinucleotide microsatellites from Loblolly pine (Pinus taeda L.) improved the recovery of trans-specific markers across related pine species.

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Undermethylated DNA as a source of microsatellites from a conifer genome.

Genome·2002

Area of Science:

  • Genetics and Genomics
  • Plant Molecular Biology
  • Conservation Genetics

Background:

  • Microsatellite markers show low conservation across vascular plant species compared to animals.
  • Developing trans-specific microsatellites in plants is crucial for population genetics and conservation.
  • Genome organization knowledge can aid in finding conserved microsatellites.

Purpose of the Study:

  • To assess the trans-specific amplification of microsatellites in hard pines (Pinus spp.).
  • To identify conserved microsatellite markers for use across diverse pine species.
  • To evaluate the utility of trinucleotide microsatellites for interspecific applications.

Main Methods:

  • Fifteen triplet-repeat microsatellites from Pinus taeda L. were tested.

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  • Amplification was attempted across seven hard pine species, one soft pine (Pinus strobus L.), and Picea rubens Sargent.
  • Analysis focused on conserved flanking regions and repeat motif composition.

    • Main Results:

    • Seven of 15 microsatellites showed trans-specific amplification in both hard and soft pine subgenera.
    • Two microsatellites (PtTX2123, PtTX3020) exhibited conserved flanking regions and repeat motifs across all tested species.
    • Perfect triplet-repeat microsatellites were more successful for trans-specific polymorphism than compound microsatellites.

    Conclusions:

    • Trinucleotide microsatellites are effective for improving trans-specific microsatellite recovery in hard and soft pines.
    • Specific microsatellites like PtTX2123 and PtTX3020 demonstrate high conservation and utility for broader pine studies.
    • The findings provide valuable markers for population genetics and conservation efforts in Pinus species.