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Related Experiment Videos

Intra-particle oxygen diffusion limitation in solid-state fermentation.

J Oostra1, E P le Comte, J C van den Heuvel

  • 1Agrotechnology and Food Sciences Department, Food and Bioprocess Engineering Group, Wageningen University, P.O. Box 8129, 6700 EV Wageningen, The Netherlands.

Biotechnology and Bioengineering
|September 6, 2001
PubMed
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Oxygen limitation in solid-state fermentation (SSF) is experimentally shown to occur in the wet layer of the fungal mat. Optimal oxygen transfer depends on gas-liquid surface area and wet layer thickness, influenced by moisture content.

Area of Science:

  • Biotechnology
  • Biochemical Engineering
  • Microbiology

Background:

  • Solid-state fermentation (SSF) is widely used, but oxygen limitation at the particle level remains poorly understood.
  • Previous studies focused on modeling oxygen transfer, lacking experimental validation.
  • Understanding intra-particle oxygen dynamics is crucial for optimizing SSF processes.

Purpose of the Study:

  • To experimentally investigate intra-particle oxygen transfer limitations in Rhizopus oligosporus cultures during SSF.
  • To identify the specific layers within the fungal mat responsible for oxygen depletion.
  • To determine factors influencing optimal oxygen transfer in SSF.

Main Methods:

  • Cultivation of Rhizopus oligosporus on defined glucose and starch media under SSF conditions.

Related Experiment Videos

  • Measurement of oxygen concentrations within the fungal mat using oxygen microelectrodes.
  • Application of a reaction-diffusion model to calculate oxygen penetration depth.
  • Analysis of oxygen consumption rates and flux at the gas-liquid interface.

    • Main Results:

    • Ethanol production indicated oxygen depletion within the fungal mat.
    • Oxygen microelectrode measurements revealed no depletion in the aerial layer but steep gradients in the wet bottom layer.
    • Oxygen became undetectable 100 microm below the gas-liquid interface after 36.5 hours, consistent with model predictions.
    • Oxygen consumption primarily occurred in the wet fungal layer, with negligible contribution from aerial hyphae.

    Conclusions:

    • Intra-particle oxygen limitation in SSF is experimentally confirmed and localized to the wet fungal mat layer.
    • Optimal oxygen transfer is dependent on the gas-liquid interfacial surface area and the thickness of the wet fungal layer.
    • Matrix moisture content significantly influences these parameters, playing a critical role in optimizing SSF oxygen transfer.