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Updated: Aug 17, 2026

In Situ Characterization of Shewanella oneidensis MR1 Biofilms by SALVI and ToF-SIMS
Published on: August 18, 2017
Structure and properties of novel inclusions in Shewanella putrefaciens
B Krause1, T J Beveridge, C C Remsen
1Center for Great Lakes Studies, University of Wisconsin-Milwaukee 53204, USA.
Abstract:
Cytoplasmic inclusions surrounded by a bilayer membrane were seen in thin sections. negatively stained and freeze-fractured preparations of Shewanella putrefaciens. Cells harvested from the late exponential and early stationary phase showed a higher number of these vesicles than bacteria isolated from early exponential or late stationary phase. Chemical dyes for polyphosphate or poly-beta-hydroxybutyrate did not stain the material enclosed within these vesicles. Elemental analysis of the material indicated that the content was organic in nature and might be a protein. HPLC analysis of the material showed that it was probably not a carbon source, nor an electron acceptor used by S. putrefaciens.
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