Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA structures and radiation injury.

G Erzgräber1, M Rosemann, K Regel

  • 1Central Institute for Molecular Biology, Berlin, Germany.

Advances in Space Research : the Official Journal of the Committee on Space Research (COSPAR)
|January 1, 1992
PubMed
Summary

This study investigated DNA damage and repair in Chinese Hamster cells using nucleoid sedimentation. Results suggest DNA subunits, not the whole DNA, are the primary targets for repair, which occurs independently within each subunit.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Can elderly patients regain their preoperative functional level after distal radius fracture type A? Results from a fracture register study using PROM.

Frontiers in surgery·2023
Same author

[Community mental health approaches for avoidance of patient placement in closed psychiatric facilities-a positioning].

Der Nervenarzt·2021
Same author

Dose-adjusted EPOCH chemotherapy with bortezomib and raltegravir for human T-cell leukemia virus-associated adult T-cell leukemia lymphoma.

Blood cancer journal·2016
Same author

Genetic background and 227Thorium as risk factors in biologically based models for induction of bone cancer in mice.

Radiation and environmental biophysics·2012
Same author

Flt3-ITD mutations in a mouse model of radiation-induced acute myeloid leukaemia.

Leukemia·2012
Same author

The effects of dietary non-starch polysaccharides on Ascaridia galli infection in grower layers.

Parasitology·2011

Area of Science:

  • Radiobiology
  • Molecular Biology
  • Genetics

Background:

  • DNA damage and repair are critical processes in radiobiology.
  • Understanding DNA subunit behavior after radiation is essential for radiobiological investigations.
  • The nuclear membrane's role in DNA repair is an area of ongoing research.

Purpose of the Study:

  • To summarize experimental results on the sedimentation behavior of damaged and restored DNA subunits attached to the nuclear membrane.
  • To investigate the effects of different radiation types (gamma-rays, neutrons, carbon ions) on DNA structure.
  • To evaluate a new DNA repair model based on subunit sensitivity and independent repair.

Main Methods:

  • Nucleoid sedimentation technique using Chinese Hamster cells (V79-4).
  • Irradiation with gamma-rays, neutrons, and carbon ions.
  • Analysis of DNA sedimentation velocity changes due to single-strand and double-strand breaks.
  • Post-irradiation incubation to study DNA rejoining and restoration.

Main Results:

  • Single-strand breaks decrease DNA sedimentation velocity by relaxing supercoiled DNA.
  • Double-strand breaks increase sedimentation velocity due to DNA fragmentation.
  • Complete restoration of DNA structures is unlikely when double-strand breaks exceed one per subunit.
  • Experimental data support a repair model where individual DNA subunits are the sensitive targets.

Conclusions:

  • DNA repair mechanisms primarily act on individual DNA subunits rather than the entire DNA molecule.
  • Repair processes for DNA damage occur independently within each DNA subunit.
  • The findings are consistent with a novel radiobiological repair model.

Related Experiment Videos