Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Endochondral bone formation in embryonic mouse pre-metatarsals.

B J Klement1, B S Spooner

  • 1Division of Biology, Kansas State University, Manhattan 66506-4901, USA.

Transactions of the Kansas Academy of Science. Kansas Academy of Science
|January 1, 1992
PubMed
Summary

Reduced gravity harms bone development. This study uses in vitro organ culture to show that early bone development stages, including cartilage formation and chondrocyte differentiation, precede bone matrix deposition, offering insights into bone physiology regulation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Skeletal tissue growth, differentiation and mineralization in the NASA rotating wall vessel.

Bone·2004
Same author

Simulated microgravity and hypergravity attenuate heart tissue development in explant culture.

Cells, tissues, organs·2000
Same author

Mineralization and growth of cultured embryonic skeletal tissue in microgravity.

Bone·1999
Same author

Liposome formation in microgravity.

Advances in space research : the official journal of the Committee on Space Research (COSPAR)·1996
Same author

Effect of microgravity and hypergravity on embryo axis alignment during postencystment embryogenesis in Artemia franciscana (Anostraca).

Journal of crustacean biology : a quarterly of the Crustacean Society for the publication of research on any aspect of the biology of crustacea·1995
Same author

In vitro chick pre-cardiac explant tissue differentiation during spaceflight on SpaceHab-02.

Acta anatomica·1995

Area of Science:

  • Skeletal Biology
  • Developmental Biology
  • Biochemistry

Background:

  • Long-term reduced gravity negatively impacts bone health.
  • Understanding early bone development is crucial for regulating mature bone physiology.
  • In vitro models can elucidate pre-deposition events in bone formation.

Purpose of the Study:

  • To characterize the in vitro development of pre-metatarsal tissue.
  • To investigate the sequential events preceding bone matrix deposition.
  • To analyze alkaline phosphatase (ALP) activity during early bone development.

Main Methods:

  • Utilized an in vitro organ culture system for pre-metatarsal tissue.
  • Cultured tissue development was compared to embryonic development.
Keywords:
NASA Discipline Cell BiologyNASA Discipline Developmental BiologyNASA Discipline Number 93-10NASA Program NSCORTNon-NASA Center

Related Experiment Videos

  • Measured alkaline phosphatase (ALP) activity throughout development.

    • Main Results:

    • Cultured pre-metatarsal tissue development mirrors embryonic development.
    • Mesenchyme differentiates and forms a cartilage rod, followed by chondrocyte differentiation.
    • Alkaline phosphatase (ALP) activity was localized and identified as the mineralizing isoform.

    Conclusions:

    • Early skeletal development stages, including cartilage formation, precede bone matrix deposition.
    • In vitro organ culture effectively models key pre-mineralization events in bone development.
    • Alkaline phosphatase (ALP) serves as a key marker for mineralizing tissues during development.