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Related Experiment Videos

Gravitational effects on mammalian cells.

G Lorenzi1, B Bechler, M Cogoli

  • 1Laboratium für Biochemie, ETH-Zentrum, Zürich, Switzerland.

The Physiologist
|January 1, 1988
PubMed
Summary

Gravitational stress differentially affects human lymphocyte activation, with higher gravity stimulating both T- and B-cells. Cell lines also exhibit unique adaptation responses to varying gravitational forces.

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Area of Science:

  • Immunology
  • Cell Biology
  • Gravitational Biology

Background:

  • Understanding how gravity affects cellular functions is crucial for space biology and medicine.
  • Lymphocyte activation plays a key role in immune responses.
  • Cellular adaptation to environmental stress is a fundamental biological process.

Purpose of the Study:

  • To investigate the impact of different gravitational forces (1xg and 10xg) on human lymphocyte activation.
  • To examine the effects of gravitational stress on various cell lines (Friend, K-562, hybridoma).
  • To determine if cells develop specific adaptation reactions to gravitational stress.

Main Methods:

  • Immunoenzymatic staining was used to assess lymphocyte activation.
  • Concanavalin A (Con A) coated to red blood cells (RBC) was employed to stimulate lymphocytes.
Keywords:
NASA Discipline Cell BiologyNASA Discipline Number 00-00NASA Program FlightNon-NASA Center

Related Experiment Videos

  • Cells were subjected to varying gravitational forces using a centrifuge and clinostat.

    • Main Results:

    • At 10xg, both T- and B-lymphocytes showed increased activation, while at 1xg, only T-cells were stimulated.
    • Chemical modification of T-cell membranes (sodium periodate) showed depressed activation at 10xg.
    • Friend, K-562, and hybridoma cell lines demonstrated distinct adaptation responses to gravitational stress.

    Conclusions:

    • Gravitational force has a differential effect on T- and B-lymphocyte activation.
    • Lymphocyte activation pathways are sensitive to changes in gravity.
    • Different cell lines exhibit unique adaptive mechanisms to gravitational challenges, highlighting cellular plasticity.