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Related Experiment Videos

Comparative performance of Colisure.

G A McFeters1, S C Broadaway, B H Pyle

  • 1Microbiology Department, Montana State University, Bozeman 59717, USA.

Journal - American Water Works Association
|September 7, 2001
PubMed
Summary
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Colisure medium effectively detects stressed total coliform bacteria and E. coli in drinking water, outperforming standard methods in sensitivity and specificity for water quality testing.

Area of Science:

  • Environmental microbiology
  • Water quality analysis
  • Microbial detection methods

Background:

  • Standard methods for detecting coliforms in drinking water can be challenged by chlorine stress.
  • Accurate enumeration of stressed bacteria is crucial for public health protection.

Purpose of the Study:

  • To evaluate the performance of Colisure presence-absence medium against standard reference methods.
  • To assess the efficacy of Colisure in detecting chlorine-stressed total coliforms and E. coli.

Main Methods:

  • Comparison of Colisure with lauryl tryptose broth (LTB) and bile green lactose broth for total coliform detection.
  • Evaluation of Colisure against LTB confirmed in EC medium with 4-methylumbelliferyl B-D-glucuronide (EC/MUG) for E. coli detection.
  • Assessment of Colisure's sensitivity and specificity on non-chlorinated samples.

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Main Results:

  • Colisure detected significantly more positive results for total coliforms compared to LTB after 24, 28, and 48 hours.
  • Colisure identified higher levels of chlorine-injured E. coli than the standard LTB/EC/MUG method.
  • Sensitivity and specificity of Colisure were high, ranging from 96 to 100% for non-chlorinated samples.

Conclusions:

  • Colisure is a sensitive and specific medium for detecting total coliforms and E. coli, even under chlorine stress.
  • Colisure offers an improved alternative to standard methods for routine drinking water monitoring.
  • The medium demonstrates robust performance in identifying viable but injured bacterial populations.