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Related Experiment Videos

A method for preserving saliva samples at ambient temperatures.

R R Nimmagudda1, R Ramanathan, L Putcha

  • 1Universities Space Research Association, Johnson Space Center, Houston, TX 77058, USA.

Biochemical Archives
|August 1, 1997
PubMed
Summary

Saliva samples remain stable for a year at room temperature with sodium benzoate and citric acid preservative (P1). This method supports biochemical studies without cold storage, preserving key analytes like acetaminophen and antipyrine.

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Area of Science:

  • Biochemistry
  • Biopharmaceutical analysis
  • Analytical chemistry

Background:

  • Cold storage is often required for saliva sample stability.
  • Limited accessibility to cold storage can hinder biochemical and biopharmaceutical studies.
  • Room temperature storage of saliva samples requires effective preservation methods.

Purpose of the Study:

  • To evaluate the long-term stability of saliva samples stored at room temperature with preservatives.
  • To compare the efficacy of two preservative mixtures (P1 and P2) for saliva analytes.
  • To determine the feasibility of room temperature saliva storage for biochemical analyses.

Main Methods:

  • Saliva samples were spiked with acetaminophen (APAP) and antipyrine (AP).
  • Two preservative mixtures, sodium benzoate/citric acid (P1) and ethyl/propyl paraben (P2), were tested.
Keywords:
NASA Center JSCNASA Discipline Regulatory Physiology

Related Experiment Videos

  • Samples were stored at room temperature and -20°C (control), with analysis for APAP, AP, melatonin, and cortisol over 1 year.

    • Main Results:

    • Preservative P1 maintained 100% stability for APAP and AP for 360 days at room temperature.
    • P1 also preserved melatonin (100%) and cortisol (95%) for 180 days at room temperature.
    • Preservative P2 showed limited stability, preserving AP and cortisol for 60 days and APAP for only 14 days.

    Conclusions:

    • Sodium benzoate and citric acid (P1) effectively preserve saliva samples at room temperature for up to 360 days for key analytes.
    • This preservation method facilitates biochemical and biopharmaceutical studies when cold storage is unavailable.
    • P1 offers a viable alternative to cold storage for long-term saliva sample preservation.