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Related Experiment Videos

A ground-based study for a shuttle BRIC experiment on gravity effects on gene expression.

A S Reddy1, Y L Kao, D L Mykles

  • 1Biology Department, Center for Engineering Infrastructure and Sciences in Space, Colorado State University, Fort Collins 80523, USA.

Advances in Space Research : the Official Journal of the Committee on Space Research (COSPAR)
|September 7, 2001
PubMed
Summary

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This study found that the Biological Research In a Canister (BRIC) experiment is feasible for investigating microgravity's effects on Arabidopsis gene regulation. Ground studies confirmed sufficient plant tissue can be recovered for molecular analysis.

Area of Science:

  • Space biology
  • Plant molecular biology
  • Genetics

Background:

  • Investigating the effects of reduced gravity on plant molecular processes is crucial for understanding life in space.
  • Arabidopsis thaliana is a model organism widely used in plant biology research.

Purpose of the Study:

  • To assess the feasibility of the Biological Research In a Canister (BRIC) experiment for studying microgravity effects on Arabidopsis at the molecular level.
  • To prepare for a space flight experiment by conducting ground-based validation studies.

Main Methods:

  • Germinating Arabidopsis seeds on Petri plates within a specific size range.
  • Utilizing nylon membranes for optimal recovery of plant material post-freezing.
  • Freezing seedlings at different ages to determine optimal tissue yield per canister.
Keywords:
NASA Discipline Plant BiologyNon-NASA Center

Related Experiment Videos

  • Extracting RNA from recovered tissue for differential gene display analysis.

    • Main Results:

    • Successfully germinated up to 20,000 seeds per 100 mm Petri plate.
    • Identified nylon membrane as the superior surface for post-freezing plant tissue recovery.
    • Determined that 20-40 g of tissue can be obtained per canister, depending on seedling age at freezing.
    • Confirmed that RNA yields from a single canister are sufficient for differential display to identify gravity-regulated genes.

    Conclusions:

    • The proposed BRIC experiment is a feasible approach for spaceflight research.
    • This methodology can yield valuable insights into microgravity's impact on plant gene regulation.
    • The study provides a validated protocol for molecular analysis of plants in microgravity environments.