Influence of simulated microgravity environmental factor on ginseng cell growth and ginseng saponin content

When ginseng (Panax ginseng C.A. Meyer) cells were subjected horizontal rotation on a clinostat, their growth and ginseng saponin content differed from those cultured in normal gravitational environments (control). Both fresh and dry weights of ginseng cells rotating on clinostat were higher than those of the control, and the difference in dry weight was particularly obvious. After 3 weeks of cultivation, saponin content in ginseng was 10% higher under the horizontal rotation treatment on the clinostat than that of the control. When ginseng cells were cultured on Ca2(+)-deprived medium and clinostatted for 3 weeks, their ginseng saponin content was almost twice of that of the control. Besides, in our experiments, the higher the Ca2+ concentration in medium, the lower the ginseng saponin content in the ginseng cells cultured.