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Updated: Oct 4, 2026

Chemical Triphosphorylation of Oligonucleotides
Published on: June 2, 2022
Oligomerization of 3'-amino-3'deoxyguanosine-5'phosphorimidazolidate on a d(CpCpCpCpC) template
M Tohidi1, W S Zielinski, C H Chen
1The Salk Institute for Biological Studies, San Diego, California 92138, USA.
Abstract:
3'-Amino-3'-deoxyguanosine-5'-phosphorimidazolidate (ImpGNH2) oligomerizes more rapidly and regiospecifically than related nucleotide derivatives on a d(CpCpCpCpC) template. The greater nucleophilicity of the amino group leads to efficient oligomerization even when the structure of the double-helical complex formed by the template and the substrate is not optimal for reaction. The use of amine-containing analogues should permit us to develop models of potentially prebiotic polymerization reactions that cannot be studied easily using natural nucleotides.
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