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Related Experiment Videos

Carbon isotope effects associated with autotrophic acetogenesis.

J T Gelwicks1, J B Risatti, J M Hayes

  • 1Department of Chemistry, Indiana University, Bloomington 47405, USA.

Organic Geochemistry
|January 1, 1989
PubMed
Summary

Acetobacterium woodii synthesizes acetate from CO2 and H2, exhibiting significant carbon kinetic isotope effects. This autotrophic acetogenesis impacts acetate

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Area of Science:

  • Biogeochemistry
  • Microbial Metabolism
  • Stable Isotope Geochemistry

Background:

  • Autotrophic acetogenesis is a key microbial process in carbon cycling.
  • Understanding the carbon isotope fractionation during acetate synthesis is crucial for interpreting sedimentary records.

Purpose of the Study:

  • To quantify the carbon kinetic isotope effects during autotrophic acetate synthesis by Acetobacterium woodii.
  • To assess the implications of these isotope effects for the carbon isotopic composition of sedimentary acetate.

Main Methods:

  • Isotopic analysis of CO2, methyl-carbon, and total acetate.
  • Cultivation of Acetobacterium woodii under autotrophic conditions (30°C).
  • Use of closed systems for complete mass balance and accounting for CO2/HCO3- partitioning.

Main Results:

  • Measured carbon kinetic isotope effects for the overall reaction (total carbonate --> total acetate) ranged from -59.0‰ to -57.2‰.
  • The weighted mean isotope effect was determined to be -58.6 ± 0.7‰.
  • No evidence for intramolecular ordering of carbon isotopes within the acetate molecule was found.

Conclusions:

  • Autotrophic acetogenesis by Acetobacterium woodii exhibits significant carbon isotope fractionation.
  • Environments with active autotrophic acetogenesis will likely show depleted carbon isotopic signatures in sedimentary acetate compared to organic carbon.
Keywords:
NASA Discipline ExobiologyNon-NASA Center

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