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Updated: Jun 28, 2026

Laboratory Simulation of an Iron(II)-rich Precambrian Marine Upwelling System to Explore the Growth of Photosynthetic Bacteria
Published on: July 24, 2016
Carbon isotopic composition of individual Precambrian microfossils
C H House1, J W Schopf, K D McKeegan
1Department of Earth and Space Sciences, IGPP Center for Astrobiology, University of California, Los Angeles 90095-1567, USA.
Ancient microorganisms from Australia and Canada show carbon isotope ratios. These findings suggest carbon fixation primarily occurred through the Calvin cycle or acetyl-CoA pathway, aligning with cyanobacteria.
Area of Science:
- Paleobiology
- Geochemistry
- Isotope Geochemistry
Background:
- Stromatolitic cherts contain fossil microorganisms from ancient Earth.
- Understanding early life's metabolic pathways is crucial for astrobiology and Earth's history.
Purpose of the Study:
- To analyze carbon isotope ratios in microfossils from the Bitter Springs and Gunflint Formations.
- To infer the carbon fixation pathways used by these ancient microorganisms.
Main Methods:
- Ion microprobe analysis of carbon isotope ratios (delta 13C(PDB)) in 30 fossil specimens.
- Morphological analysis to suggest phylogenetic affinities.
Main Results:
- Carbon isotope values for Bitter Springs microfossils ranged from -21.3% to -31.9%.
- Carbon isotope values for Gunflint microfossils ranged from -32.4% to -45.4%.
- Results are consistent with Calvin cycle or acetyl-CoA pathway, but not 3-hydroxypropionate or reductive TCA cycles.
Conclusions:
- The studied microfossils likely utilized the Calvin cycle or acetyl-CoA pathway for carbon fixation.
- Morphological and isotopic data support an affinity to cyanobacteria.
- Excludes the 3-hydroxypropionate and reductive tricarboxylic acid cycles as primary carbon fixation pathways for these organisms.
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