Related Experiment Video
Updated: Jul 14, 2026

Generation of Human Alloantigen-specific T Cells from Peripheral Blood
Published on: November 21, 2014
TCR usage in naive and committed alloreactive cells: implications for the understanding of TCR biases in
M Guillet1, F Sebille, J Soulillou
1Institut National de la Santé et de la Recherche Médicale (INSERM), Unité 437, Immunointervention dans les Allo- et les Xéno-transplantations, 30 Boulevard Jean Monnet, 44093 Nantes 01, Cedex, France.
Abstract:
The direct pathway of allorecognition is involved in acute allograft rejection and is characterised by TCR-mediated recognition of the MHC framework; this is thought to occur in a peptide-dependent but not peptide-specific manner. In contrast, the indirect pathway is restricted to the recipient's own MHC molecules and prevails in chronic rejection. In this pathway, the peptide has a major influence on the TCR recognition and selects alloreactive T cells with altered TCR Vbeta usage. However, qualitative analysis of Vbeta usage alone might limit our understanding of alloreactivity. The advantages of a combined quantitative assessment of Vbeta mRNA usage are discussed.
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