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Updated: Jul 19, 2026

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes
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The role of IFN-gamma in the production of Th2 subpopulations: implications for variable Th2-mediated pathologies in

A Wensky1, M C Marcondes, J J Lafaille

  • 1Division of Molecular Pathogenesis, Skirball Institute of Biomolecular Medicine, Department of Pathology, New York University Medical Center, New York, NY 10016, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|September 7, 2001
PubMed

It has become increasingly apparent in studies of mutant mice and observations of disease that cytokine production by fully committed effector T cells within the Th1 and Th2 phenotype can vary within each group. This can potentially influence the type and effectiveness of a given immune response. The factors responsible for inducing variable Th1 and Th2 subtype responses have not been well established. Using transgenic mice expressing the myelin basic protein-specific TCR, we demonstrate here that two distinct populations of Th2 cells that are characterized primarily by differential IL-4 and IL-5 expression levels can be generated depending upon the levels of IFN-gamma present at the time of priming. We also demonstrate that populations expressing high levels of IL-4 relative to IL-5 vs those with intermediate levels of IL-4 relative to IL-5 are stable and possess distinct effector functions in an experimental autoimmune encephalomyelitis model.

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