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Related Experiment Videos

Perfluorochemical liquids modulate cell-mediated inflammatory responses.

B Nakstad1, M R Wolfson, T H Shaffer

  • 1Department of Pediatrics Ullevål University Hospital, Oslo, Norway.

Critical Care Medicine
|September 8, 2001
PubMed
Summary
This summary is machine-generated.

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Perfluorochemical liquids (PFCs) like perflubron (PFB) and perfluorodecalin (PFD) did not significantly induce inflammation in blood leukocytes at concentrations used in liquid ventilation. However, high PFB concentrations increased monocyte reactive oxygen species, and both PFCs showed anti-inflammatory effects with lipopolysaccharide.

Area of Science:

  • Biomedical Engineering
  • Immunology
  • Toxicology

Background:

  • Perfluorochemical liquids (PFCs) are used in liquid ventilation.
  • Understanding their inflammatory potential is crucial for patient safety.

Purpose of the Study:

  • To investigate the inflammatory responses of blood leukocytes to two different PFCs: perfluorodecalin (PFD) and perflubron (PFB).
  • To assess the effects of PFCs on leukocyte adhesion molecules, reactive oxygen species production, and cytokine release.

Main Methods:

  • Whole blood from healthy adults was incubated with varying concentrations of PFD and PFB, with and without bacterial lipopolysaccharide.
  • Leukocyte responses were measured using flow cytometry for adhesion molecules (CD62L, CD11b) and reactive oxygen species, and ELISA for cytokines (interleukin-8, tumor necrosis factor-alpha).

Related Experiment Videos

  • Electron microscopy was used to examine cellular morphology.

    • Main Results:

    • PFB, at high concentrations, increased reactive oxygen species in monocytes. PFD did not induce significant inflammatory changes in resting leukocytes.
    • PFB increased CD11b expression in monocytes and granulocytes. PFD caused a minor increase in interleukin-8 secretion.
    • In the presence of lipopolysaccharide, neither PFC induced significant pro-inflammatory cytokine production; instead, a trend for decreased tumor necrosis factor-alpha was observed.

    Conclusions:

    • PFCs at concentrations relevant to liquid ventilation do not appear to induce significant inflammation in blood leukocytes.
    • PFB and PFD can elicit specific cellular responses (ROS, CD11b expression, IL-8 secretion) at higher concentrations.
    • PFCs may attenuate lipopolysaccharide-induced tumor necrosis factor-alpha production, suggesting a potential anti-inflammatory role in certain contexts.