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Collagenolytic activity of a cell wall preparation from Fusobacterium necrophorum subsp. necrophorum
K Okamoto1, M Kanoe, T Watanabe
1Laboratory of Animal Health, Tokyo University of Agriculture, Kanagawa, Japan.
Microbios
|September 8, 2001
Abstract:
A collagenolytic preparation of Fusobacterium necrophorum subsp. necrophorum was derived from the bacterial cell. It was further treated for gel permeation with Toyopearl HW 50, followed by Sepharose 4B column chromatography. In sodium dodecyl sulphate polyacrylamide gel electrophoresis, the final preparation exhibited one definite band and at least one faint band. It was inactivated completely by adjusting the pH to 4.0 or by heating at 80 degrees C for 30 min.