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Mediated microbial biosensor using a novel yeast strain for wastewater BOD measurement
S P Trosok1, B T Driscoll, J H Luong
1Biotechnology Research Institute, National Research Council Canada, Montreal, Quebec.
Applied Microbiology and Biotechnology
|September 11, 2001
Summary
New yeast biosensors (SPT1 and SPT2) were developed for measuring biochemical oxygen demand (BOD). The SPT1 strain demonstrated superior performance, enabling accurate BOD estimation in various effluent samples.
Area of Science:
- Environmental Science
- Biotechnology
- Electrochemistry
Background:
- Accurate measurement of biochemical oxygen demand (BOD) is crucial for monitoring water quality.
- Development of sensitive and rapid biosensors can improve environmental monitoring efficiency.
Purpose of the Study:
- To develop novel microbial biosensors for the estimation of biochemical oxygen demand (BOD).
- To evaluate the performance of isolated yeast strains for BOD sensing applications.
Main Methods:
- Isolation and immobilization of two new yeast strains (SPT1 and SPT2) on glassy carbon electrodes.
- Utilization of ferricyanide as an electron mediator with glucose/glutamic acid (GGA) as a substrate.
- Optimization and validation of the biosensor for BOD measurements.
Main Results:
- The yeast strain SPT1 exhibited a 3-fold greater metabolic assimilation capability and enhanced responses compared to SPT2.
- The developed biosensor achieved an extended linear range of 2-100 mg/l for BOD estimation using GGA standards.
- The sensor demonstrated a BOD detection limit of 2 mg/l and a rapid response time of 5 minutes for pulp mill effluents.
- Response reproducibility was found to be +/-10% for a 10 mg BOD/l GGA standard.
Conclusions:
- The novel yeast-based microbial biosensor, particularly using the SPT1 strain, offers a sensitive and efficient method for BOD estimation.
- This biosensor technology shows promise for effective monitoring of water quality, especially in industrial effluent analysis.