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[Kininases from yeasts (author's transl)].

G Fischer, U Eckloff

    Zentralblatt Fur Bakteriologie, Parasitenkunde, Infektionskrankheiten Und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie Und Parasitologie
    |January 1, 1975
    PubMed
    Summary
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    This study investigated kinin-inactivating enzymes, known as kininases, in Candida yeast strains. Environmental strains showed higher kininase activity than those from disease sites, indicating species-specific differences.

    Area of Science:

    • Microbiology
    • Enzymology
    • Biochemistry

    Background:

    • Limited research exists on kinin-decomposing enzymes in yeasts.
    • Candida species are known human pathogens and environmental inhabitants.
    • Kininases play a role in regulating kinin peptides involved in physiological processes.

    Purpose of the Study:

    • To investigate the presence and activity of kinin-inactivating enzymes (kininases) in various Candida yeast strains.
    • To compare kininase activity between Candida strains isolated from disease foci and environmental sources.
    • To characterize the nature of the identified kinin-decomposing enzymes.

    Main Methods:

    • Qualitative and quantitative analysis of kinin-inactivating enzyme activity.
    • Use of intact washed cell suspensions, cell-homogenized blastospores, nutrient media, and supernatant preparations.

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  • Bradykinin inactivation assay using isolated guinea-pig ileum in a water-bath test.
  • Enzyme characterization through treatment with 1,10 phenanthroline, acid, and heat.

    • Main Results:

    • Intact washed cell suspensions of some Candida strains from disease foci exhibited bradykinin inactivation.
    • Environmental Candida strains (e.g., Candida tropicalis E2, Rhodotorula rubra H14) showed significant kininase activity, enhanced by cell homogenization.
    • Kininase activity was detected in nutrient media for Candida tropicalis E2 but not in supernatants.
    • Enzyme inactivation was prevented by specific inhibitors, confirming the presence of kininases.

    Conclusions:

    • Species-specific differences in kininase presence and activity exist among Candida strains.
    • Environmental Candida strains may possess higher kininase activity compared to those from disease sites.
    • The identified enzymes are confirmed to be kininases, suggesting potential roles in yeast biology and host-pathogen interactions.