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Effect of pH and Zn(2+) on subcultured muscle cells from Macrobrachium nipponense
1College of Life Sciences, Hebei University, 1. Hezuo Road, Boading, Hebei 071002, P. R. China. Wangwn@mail.hbu.edu.cn
Abstract:
A cell culture system was devised for muscle cell of Macrobrachium nipponense in the study. The juvenile and adult shrimps were held in laboratory aquaria with penicillin 1000 IU/ml and streptomycin 1000 microg/ml for 12-24 hours. Cell cultures were established in medium 199 supplemented with 20% fetal bovine serum, 1 g/L glucose, 5.2 g/L NaCl, 1.43 g/L CaCl(2), 0.05 g/L MgCl(2), 100 IU/mL penicillin and 100 microg/ml streptomycin. Fibroblast-like cells were passaged up to three times and survived for 54 days. The results showed the optimum for subculture in vitro was in medium 199 with pH 7.6. Moreover, basal medium supplemented with Zn(2+) 60 microg/L could enhance the growth of the muscle cells. It was found that better results for cell culture would be obtained more easily with juvenile shrimps caught in spring than adults in summer or autumn; and shrimps caught within 12 hours after ecdysis could grow much better than the intermoult shrimps.
Insights
This study developed a cell culture system for Macrobrachium nipponense muscle cells. Optimal conditions were identified, including specific media and shrimp collection timing, for successful in vitro cell growth.
Area of Science:
- Aquaculture
- Cell Biology
- Invertebrate Zoology
Background:
- Muscle cell culture is crucial for understanding shrimp physiology.
- Developing reliable cell lines is essential for aquaculture research.
Purpose of the Study:
- To establish a novel cell culture system for Macrobrachium nipponense muscle cells.
- To determine optimal conditions for in vitro cell growth and survival.
Main Methods:
- Muscle cells from Macrobrachium nipponense were cultured using Medium 199.
- Antibiotics (penicillin and streptomycin) were used for sterilization.
- Cell viability and growth were monitored under various conditions.
Main Results:
- Fibroblast-like cells were successfully passaged and survived for 54 days.
- Optimal subculture conditions included Medium 199 at pH 7.6.
- Supplementation with Zn(2+) at 60 microg/L enhanced cell growth.
- Juvenile shrimp caught in spring and recently molted shrimp yielded better culture results.
Conclusions:
- A viable cell culture system for Macrobrachium nipponense muscle cells was established.
- Specific environmental and physiological factors significantly impact cell culture success.
- This system provides a foundation for further research in shrimp muscle biology and aquaculture.