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Activity changes in mouse brain histidine decarboxylase.

R W Schayer, M A Reilly

    Agents and Actions
    |May 1, 1975
    PubMed
    Summary
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    Histidine decarboxylase activity in mouse brains fluctuates significantly with various factors. Protein synthesis inhibition reduced enzyme activity, while infection appeared to stimulate it.

    Area of Science:

    • Neuroscience
    • Biochemistry
    • Enzymology

    Background:

    • Histidine decarboxylase (HDC) is a key enzyme in histamine synthesis.
    • Understanding HDC regulation is crucial for comprehending brain histamine's physiological roles.

    Purpose of the Study:

    • To investigate the influence of diverse factors on brain histidine decarboxylase activity in mice.
    • To explore the impact of protein synthesis inhibition and infection on HDC levels.

    Main Methods:

    • Assessed HDC activity in whole brain and regional brain samples from mice.
    • Varied factors including age, sex, feeding status, season, time of day, and freezing.
    • Administered intracerebral injections of protein synthesis inhibitors (acetoxycycloheximide) over 2-5 days.
    • Observed HDC activity changes in response to experimental stimuli, including infection.

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    Main Results:

    • Acetoxycycloheximide treatment for 5 days significantly reduced brain HDC activity.
    • Brain infection was associated with a marked increase in HDC activity in one experimental instance.
    • Normal mouse brain enzyme activity exhibited substantial fluctuations over extended periods (months to a year).

    Conclusions:

    • Brain histidine decarboxylase activity is modulated by physiological and pathological factors.
    • Protein synthesis inhibition impacts HDC levels, suggesting a role for protein turnover in its regulation.
    • The observed long-term fluctuations in HDC activity suggest complex, yet unidentified, physiological regulatory mechanisms.