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Updated: Aug 11, 2026

Detection of Antibodies That Neutralize the Cellular Uptake of Enzyme Replacement Therapies with a Cell-based Assay
Published on: September 10, 2018
EDTA-induced pseudo-neutropenia resolved with kanamycin
1Department of Clinical Laboratories, Haematology Division, Catharina Hospital, Eindhoven, The Netherlands.
Abstract:
This report describes a case of spurious neutropenia caused by EDTA-dependent in vitro agglutination of neutrophils. After raising the temperature of the sample to 37 degrees C the agglutination was irreversible, but it resolved completely after addition of kanamycin. Previously this method has been shown to be effective in EDTA-dependent pseudo-thrombocytopenia, but this is the first report demonstrating successful application in EDTA-dependent pseudo-neutropenia.
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