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Related Experiment Videos

Predictable tissue shrinkage during frozen section histopathologic processing for Mohs micrographic surgery.

E S Gardner1, W T Sumner, J L Cook

  • 1Division of Dermatology, Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA.

Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [Et Al.]
|September 13, 2001
PubMed
Summary

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Mohs surgery skin specimens shrink significantly during frozen section processing, averaging 11.6% shorter. This shrinkage, particularly on the trunk and extremities, is crucial for surgeons to consider when assessing surgical margins.

Area of Science:

  • Dermatology
  • Surgical Pathology
  • Histotechnology

Background:

  • Mohs micrographic surgery requires precise examination of entire surgical margins.
  • Discrepancies in specimen size can arise from tissue shrinkage during processing or incomplete specimens.
  • The extent of skin shrinkage during frozen section processing has not been previously quantified.

Purpose of the Study:

  • To quantify the degree of tissue shrinkage in Mohs micrographic surgical specimens.
  • To assess shrinkage following routine frozen section processing and hematoxylin and eosin staining.

Main Methods:

  • Prospective enrollment of 117 Mohs surgery patients (135 cutaneous tumors).
  • Measurement of surgical specimens at multiple stages: post-excision, post-freezing, post-slide placement, and post-staining.

Related Experiment Videos

  • Statistical analysis to determine the significance of size discrepancies.

    • Main Results:

    • Skin specimens showed statistically significant length alterations after processing.
    • Average shrinkage was 11.6% compared to post-excision measurements.
    • Greater shrinkage observed in specimens from the trunk/extremities (16.3%) versus head/neck (10.2%).

    Conclusions:

    • Skin specimens shrink during frozen section processing, supporting anecdotal evidence.
    • Understanding this shrinkage allows Mohs surgeons to interpret smaller specimens with greater confidence regarding complete margin assessment.
    • This quantification aids in differentiating processing-induced shrinkage from incomplete tissue sampling.