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Updated: Oct 4, 2026

Chemical Triphosphorylation of Oligonucleotides
Published on: June 2, 2022
Control of reaction chemoselectivity with a circular DNA template in the ligation of short oligodeoxyribonucleotides
1American Radiolabeled Corporation, St. Louis, MO 63146, USA.
Abstract:
A study has been conducted to examine the chemoselective control attained in the ligation of short oligodeoxyribonucleotides (ODNs) directed by association on a circular DNA template through triplex formation relative to the same ligation on a single-strand DNA template through duplex formation. The highly efficient, chemospecific ligation achieved under a variety of conditions on the circular DNA template presumably arises from the higher substrate-template association resulting in improved transition state accessibility and protection of the ODNs from side reactions.
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