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Epitope mapping of neutralizing botulinum neurotoxin A antibodies by phage display
B P Mullaney1, M G Pallavicini, J D Marks
1Department of Laboratory Medicine, University of California at San Francisco, San Francisco, California 94143, USA. mullaney@cc.ucsf.edu
Infection and Immunity
|September 13, 2001
Abstract:
Single-chain antibodies neutralize activity and bind nonoverlapping epitopes of botulinum A neurotoxin. Two phage display epitope libraries were constructed from the 1.3 kb of binding domain cDNA. The minimal epitopes selected against the single-chain Fv-Fc antibodies correspond to conformational epitopes with amino acid residues 1115 to 1223 (S25), 1131 to 1264 (3D12), and 889 to 1294 (C25).