Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

ASH1 mRNA localization in three acts.

D L Beach1, K Bloom

  • 1Department of Biology, University of North Carolina, Chapel Hill, NC 27599-3280, USA. dbeach@email.unc.edu

Molecular Biology of the Cell
|September 13, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Search for dark matter produced in association with heavy-flavor quark pairs in proton-proton collisions at <math> </math>.

The European physical journal. C, Particles and fields·2020
Same author

Extraction and validation of a new set of CMS pythia8 tunes from underlying-event measurements.

The European physical journal. C, Particles and fields·2020
Same author

Searches for physics beyond the standard model with the <math></math> variable in hadronic final states with and without disappearing tracks in proton-proton collisions at <math> </math>.

The European physical journal. C, Particles and fields·2020
Same author

Search for Physics beyond the Standard Model in Events with Overlapping Photons and Jets.

Physical review letters·2020
Same author

Measurements of triple-differential cross sections for inclusive isolated-photon+jet events in <math></math> collisions at <math> </math>.

The European physical journal. C, Particles and fields·2019
Same author

Measurement of the average very forward energy as a function of the track multiplicity at central pseudorapidities in proton-proton collisions at <math> </math>.

The European physical journal. C, Particles and fields·2019
Same journal

Csf1 facilitates adaptive membrane lipid remodeling linked to ER-plasma membrane contact sites.

Molecular biology of the cell·2026
Same journal

Differential effects of tropomyosin paralogs on mitochondrial dynamics in <i>Saccharomyces cerevisiae</i>.

Molecular biology of the cell·2026
Same journal

Mutating different α-tubulin acetylation sites has distinct effects on axon terminal morphogenesis in <i>Drosophila melanogaster</i>.

Molecular biology of the cell·2026
Same journal

Novel KIF22 Variants Disrupt Mitosis in Human Chondrocytes and Expand SEMDJL2 Mechanisms.

Molecular biology of the cell·2026
Same journal

Functions and trafficking mechanisms of RIC-8 in <i>C. elegans</i> and mammalian cilia.

Molecular biology of the cell·2026
Same journal

The <i>C. elegans</i> WASH complex supports epithelial polarity by promoting endosomal sorting of E-Cadherin.

Molecular biology of the cell·2026
See all related articles

Novel green fluorescent protein (GFP) techniques reveal yeast mRNA localization phases: packaging, transport, and docking. Specific proteins control ASH1 mRNA positioning, but mRNA localization doesn't always dictate protein localization.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • mRNA localization is crucial for spatial protein synthesis in eukaryotes.
  • Budding yeast provides a model system to study asymmetric cell division and mRNA transport.

Purpose of the Study:

  • To elucidate the mechanisms and protein requirements for specific mRNA localization in yeast.
  • To investigate the relationship between mRNA localization and the localization of its translated protein product.

Main Methods:

  • Utilized novel green fluorescent protein (GFP) labeling techniques to track specific mRNA transcripts in vivo.
  • Employed mutant strains to identify proteins involved in mRNA packaging, transport, and tethering.
  • Examined the protein localization of Yta6p, a mother-specific protein, relative to its localized mRNA.

Related Experiment Videos

Main Results:

  • Identified discrete phases of mRNA localization: packaging, transport, and docking.
  • Discovered three functional categories of 'She' proteins essential for ASH1 mRNA localization.
  • Demonstrated that mRNA localization to the bud does not guarantee protein localization to the bud, as Yta6p remained in the mother cell.

Conclusions:

  • The precise localization of ASH1 mRNA to the bud tip requires a coordinated effort of specific protein complexes.
  • mRNA localization is a regulated process with distinct stages, involving specific protein machinery.
  • mRNA localization does not always determine the final subcellular localization of the translated protein, suggesting post-transcriptional or post-translational regulatory mechanisms.