Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Osteoblast cell death on methacrylate polymers involves apoptosis.

J E Gough1, S Downes

  • 1School of Biomedical Sciences, E Floor, University of Nottingham, Medical School, Queen's Medical Centre, Nottingham, NG7 2UH, United Kingdom. j.gough@ic.ac.uk

Journal of Biomedical Materials Research
|September 13, 2001
PubMed
Summary

Methacrylate monomers, including tetrahydrofurfuryl methacrylate, induce apoptosis in human osteoblasts. Pre-incubation of polymers enhances biocompatibility, reducing cell death at the implant-tissue interface.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

3D bioprinting of an intervertebral disc tissue analogue with a highly aligned annulus fibrosus via suspended layer additive manufacture.

Biofabrication·2024
Same author

Resistance Bioassays and Allele Characterization Inform Analysis of Spodoptera frugiperda (Lepidoptera: Noctuidae) Introduction Pathways in Asia and Australia.

Journal of economic entomology·2022
Same author

Determinants of Insecticide Resistance Evolution: Comparative Analysis Among Heliothines.

Annual review of entomology·2022
Same author

High nucleotide diversity and limited linkage disequilibrium in Helicoverpa armigera facilitates the detection of a selective sweep.

Heredity·2015
Same author

Schwann cell interactions with polymer films are affected by groove geometry and film hydrophilicity.

Biomedical materials (Bristol, England)·2014
Same author

Differentiated adipose-derived stem cells act synergistically with RGD-modified surfaces to improve neurite outgrowth in a co-culture model.

Journal of tissue engineering and regenerative medicine·2013

Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Dental Implantology

Background:

  • Implant success hinges on the implant-tissue interface.
  • Tissue necrosis is a significant cause of implant failure.
  • Understanding cellular responses to biomaterials is crucial for preventing implant complications.

Purpose of the Study:

  • To investigate apoptosis in primary human osteoblasts exposed to methacrylate polymers.
  • To compare the cytotoxicity of poly(ethyl methacrylate)/tetrahydrofurfuryl methacrylate and poly(methyl methacrylate).
  • To assess the impact of leachable monomers and pre-incubation on polymer biocompatibility.

Main Methods:

  • In vitro culture of primary human osteoblasts on methacrylate polymer discs.
  • Exposure to leachable monomers and varying concentrations of monomers.

Related Experiment Videos

  • Assessment of cell death via apoptosis.
  • Evaluation of polymer biocompatibility after pre-incubation in serum-containing medium.

    • Main Results:

    • Both leachable and direct monomer exposure induced apoptosis in osteoblasts.
    • Tetrahydrofurfuryl methacrylate monomer demonstrated higher toxicity than methyl methacrylate.
    • Pre-incubation of polymers in serum-containing medium improved biocompatibility.
    • Polymers used immediately after polymerization showed high apoptosis levels, reduced after heat treatment.

    Conclusions:

    • Methacrylate monomers and leached components can induce apoptosis in osteoblasts.
    • Tetrahydrofurfuryl methacrylate is more cytotoxic than methyl methacrylate.
    • Post-polymerization treatment (heat incubation) and pre-incubation in serum can enhance polymer biocompatibility.
    • Apoptosis at the implant site in vivo is a potential concern.